1ETQ
THE CRYSTAL STRUCTURE OF E. COLI FIS MUTANT R71Y
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-06-28 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.210, 57.970, 135.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.780 - 2.800 |
| Rwork | 0.216 |
| R-free | 0.27700 |
| Starting model (for MR) | The wild-type FIS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 18.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.103 * | 0.510 * |
| Total number of observations | 61806 * | |
| Number of reflections | 10232 | |
| <I/σ(I)> | 20.3 | |
| Completeness [%] | 99.5 | 99.8 |
| Redundancy | 6 | 3.97 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 10 mg/ml protein, 0.5 M sodium chloride, 0.05 M Na-Hepes(pH7.5), 12.5% PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | 1 (M) | ||
| 3 | 1 | drop | Tris-HCl | 20 (mM) | |
| 4 | 1 | reservoir | Na-HEPES | 0.1 (M) | |
| 5 | 1 | reservoir | PEG8000 | 25 (%) |
