1ET1
CRYSTAL STRUCTURE OF HUMAN PARATHYROID HORMONE 1-34 AT 0.9 A RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 1998-08-10 |
| Detector | MARRESEARCH |
| Spacegroup name | P 65 |
| Unit cell lengths | 30.177, 30.177, 110.435 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 13.500 - 0.900 |
| R-factor | 0.137 |
| Rwork | 0.137 |
| R-free | 0.14000 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 16.628 * |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 13.500 | 0.930 |
| High resolution limit [Å] | 0.900 | 0.900 |
| Rmerge | 0.054 | 0.163 |
| Total number of observations | 111749 * | |
| Number of reflections | 37771 | |
| <I/σ(I)> | 8.2 | |
| Completeness [%] | 90.5 | 75.5 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 20 * | 2.5 M ammonium sulfate, 5% isopropanol and 0.1 M sodium acetate buffer, pH 4.5., VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | glycerol | 20 (%) | |
| 3 | 1 | reservoir | ammonium sulfate | 2.5 (M) | |
| 4 | 1 | reservoir | isopropanol | 5 (%) | |
| 5 | 1 | reservoir | sodium acetate | 0.1 (M) |
