1ET0
CRYSTAL STRUCTURE OF AMINODEOXYCHORISMATE LYASE FROM ESCHERICHIA COLI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 287 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-01-20 |
| Detector | FUJI |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 40.500, 73.930, 83.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.200 |
| R-factor | 0.204 |
| Rwork | 0.196 |
| R-free | 0.27900 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.425 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.072 | 0.135 |
| Total number of observations | 88073 * | |
| Number of reflections | 12330 | |
| <I/σ(I)> | 30.9 | |
| Completeness [%] | 92.2 | 85 |
| Redundancy | 7.14 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | drop consists of equal volume of protein and reservoir solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | PLP | 0.1 (mM) | |
| 3 | 1 | drop | potassium phosphate | 20 (mM) | |
| 4 | 1 | reservoir | PEG6000 | 17 (%) | |
| 5 | 1 | reservoir | 2-propanol | 17 (%) | |
| 6 | 1 | reservoir | citrate | 100 (mM) |
