1ESC

THE MOLECULAR MECHANISM OF ENANTIORECOGNITION BY ESTERASES

Experimental procedure

Spacegroup name	C 1 2 1
Unit cell lengths	131.025, 48.501, 70.307
Unit cell angles	90.00, 118.09, 90.00

Refinement procedure

Resolution	7.500 - 2.080 *
R-factor	0.16 *
Rwork	0.166
RMSD bond length	0.011 *
RMSD bond angle	0.046 *
Phasing software	X-PLOR
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.080 *
Rmerge	0.070 *
Number of reflections	19449 *
Completeness [%]	84.0 *
Redundancy	4.2 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.6 *		Green, R., (1992) J. Mol. Biol., 227, 569. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	ammonium sulfate	45 (%sat)
3	1	drop	potassium phosphate	100 (mM)
4	1	reservoir	ammonium sulfate	45 (%sat)
5	1	reservoir	potassium phosphate	100 (mM)