1ESB

DIRECT STRUCTURE OBSERVATION OF AN ACYL-ENZYME INTERMEDIATE IN THE HYDROLYSIS OF AN ESTER SUBSTRATE BY ELASTASE

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	52.020, 57.220, 74.660
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.300
R-factor	0.21
Rwork	0.210
RMSD bond length	0.018 *
RMSD bond angle	3.000 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	11.000 *
High resolution limit [Å]	2.300 *
Number of reflections	10437 *
Completeness [%]	97.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	5 *	2 *	Sawyer, L., (1978) J. Mol. Biol., 118, 137. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	sodium sulfate	1 (M)
2	1	1	protein	20 (mg/ml)
3	1	1	sodium acetate	10 (mM)