1ES8
Crystal structure of free BglII
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-03-01 |
| Detector | BRANDEIS - B4 |
| Wavelength(s) | 0.9793, 0.9789, 0.9689 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 79.150, 79.150, 66.520 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| Rwork | 0.214 |
| R-free | 0.25800 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.270 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.057 * | 0.138 * |
| Total number of observations | 33794 * | |
| Number of reflections | 10979 | |
| <I/σ(I)> | 14.4 | |
| Completeness [%] | 99.4 | 99.1 |
| Redundancy | 3.1 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 * | 4 * | drop consists of equal amounts of protein and precipitant solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | drop | glycerol | 10 (%(v/v)) | |
| 5 | 1 | drop | dithiothreitol | 1 (mM) | |
| 6 | 1 | reservoir | isopropanol | 30 (%(v/v)) | precipitant |
| 7 | 1 | reservoir | sodium acetate | 0.2 (M) | precipitant |
| 8 | 1 | reservoir | Bis-tris | 100 (mM) | precipitant |
