1ES7

COMPLEX BETWEEN BMP-2 AND TWO BMP RECEPTOR IA ECTODOMAINS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1999-08-12
Detector	SIEMENS X1000
Spacegroup name	P 65
Unit cell lengths	109.330, 109.330, 101.905
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	100.000 - 2.900
Rwork	0.191
R-free	0.22920
RMSD bond length	0.007
RMSD bond angle	25.050 *
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	16.000	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.098	0.385
Number of reflections	15184 *
<I/σ(I)>	10.2	2.4
Completeness [%]	98.6	99.5
Redundancy	3.45	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6 *	296	sodium acetate, imidazole, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
2	1	drop	MES	20 (mM)
3	1	drop		0.7 (M)
4	1	reservoir	sodium acetate	0.75 (M)
5	1	reservoir	imidazole	0.1 (mM)