1ES1
CRYSTAL STRUCTURE OF VAL61HIS MUTANT OF TRYPSIN-SOLUBILIZED FRAGMENT OF CYTOCHROME B5
Replaces: 1QDXExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1998-01-01 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 70.757, 40.433, 39.306 |
Unit cell angles | 90.00, 111.88, 90.00 |
Refinement procedure
Resolution | 12.000 - 2.100 |
Rwork | 0.192 |
R-free | 0.24400 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.150 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 12.000 | 2.150 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.090 | 0.232 |
Number of reflections | 5579 | |
<I/σ(I)> | 8.3 | |
Completeness [%] | 91.7 | 79.5 |
Redundancy | 1.93 | 1.62 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 20 * | phosphate buffer, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | phosphate | 3.2 (M) | pH8.0 |
2 | 1 | drop | protein | 20 (mg/ml) |