1ERX
CRYSTAL STRUCTURE OF NITROPHORIN 4 COMPLEXED WITH NO
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR571 |
| Temperature [K] | 140 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1999-04-29 |
| Detector | ENRAF-NONIUS FAST |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 70.319, 42.636, 52.573 |
| Unit cell angles | 90.00, 94.19, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.400 |
| R-factor | 0.17 |
| Rwork | 0.170 |
| R-free | 0.24000 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.039 |
| Data reduction software | MADNESS |
| Data scaling software | SCALA |
| Phasing software | X-PLOR |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.480 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.110 | 0.300 |
| Total number of observations | 56842 * | |
| Number of reflections | 28023 | |
| <I/σ(I)> | 9.9 | |
| Completeness [%] | 91.0 | 79 |
| Redundancy | 2 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 295 | drop consists of 1:1 mixture of well and protein solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | |
| 3 | 1 | reservoir | PEG4000 | 50 (%(w/v)) | |
| 4 | 1 | reservoir | sodium citrate | 100 (mM) | |
| 5 | 1 | reservoir | argon |
