1ERJ
CRYSTAL STRUCTURE OF THE C-TERMINAL WD40 DOMAIN OF TUP1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 296 |
Detector technology | IMAGE PLATE |
Collection date | 1997-09-30 |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | P 31 |
Unit cell lengths | 119.280, 119.280, 77.070 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.300 |
Rwork | 0.228 |
R-free | 0.26600 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.620 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.083 | 0.308 |
Number of reflections | 53856 | |
<I/σ(I)> | 9.8 | |
Completeness [%] | 98.8 | 95.9 |
Redundancy | 2.9 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 20 * | PEG 6000, Bis-Tris Propane, NaCl, DTT, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | bis-tris-propane | 50-100 (mM) | |
2 | 1 | reservoir | 50 (mM) | ||
3 | 1 | reservoir | PEG6000 | 23-26 (%(w/v)) | |
4 | 1 | reservoir | dithiothreitol | 2 (mM) | or 1 mM BMS |