1ERJ

CRYSTAL STRUCTURE OF THE C-TERMINAL WD40 DOMAIN OF TUP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	296
Detector technology	IMAGE PLATE
Collection date	1997-09-30
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 31
Unit cell lengths	119.280, 119.280, 77.070
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.300
Rwork	0.228
R-free	0.26600
RMSD bond length	0.009
RMSD bond angle	1.620
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.083	0.308
Number of reflections	53856
<I/σ(I)>	9.8
Completeness [%]	98.8	95.9
Redundancy	2.9	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	20 *	PEG 6000, Bis-Tris Propane, NaCl, DTT, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	bis-tris-propane	50-100 (mM)
2	1	reservoir		50 (mM)
3	1	reservoir	PEG6000	23-26 (%(w/v))
4	1	reservoir	dithiothreitol	2 (mM)	or 1 mM BMS