1ERI

X-RAY STRUCTURE OF THE DNA-ECO RI ENDONUCLEASE-DNA RECOGNITION COMPLEX: THE RECOGNITION NETWORK AND THE INTEGRATION OF RECOGNITION AND CLEAVAGE

Replaces:  1R1E

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Spacegroup name	P 3 2 1
Unit cell lengths	118.400, 118.400, 49.700
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.700 *
R-factor	0.17
Rwork	0.170
R-free	0.28000
RMSD bond length	0.006
RMSD bond angle	1.580
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.700
Number of reflections	8775
Completeness [%]	60.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *		4 *	Grable J., (1984) J. Biomol. Struct. Dyn., 1, 1149. *

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	WATER
2	1	1	PEG 400
3	1	2	WATER
4	1	2	PEG 400

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	2.7 (mg/ml)
2	1	drop	DNA	2.8 (mg/ml)
3	1	drop	PEG400	8 (%(w/v))
4	1	reservoir	Bis-Tris propane	40 (mM)
5	1	reservoir	EDTA	1 (mM)
6	1	reservoir	PEG400	16 (%)