1EQZ
X-RAY STRUCTURE OF THE NUCLEOSOME CORE PARTICLE AT 2.5 A RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-04-18 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 105.280, 109.710, 181.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 * |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.045 * |
| Total number of observations | 605950 * |
| Number of reflections | 72200 * |
| Completeness [%] | 98.7 |
| Redundancy | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6 | pH 6.0, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | 50 (mM) | ||
| 2 | 1 | 1 | potassium cacodylate | 10 (mM) | |
| 3 | 1 | 1 | PMSF | 1 (mM) | |
| 4 | 1 | 1 | high concentration | ||
| 5 | 1 | 2 | 50 (mM) | ||
| 6 | 1 | 2 | potassium cacodylate | 10 (mM) | |
| 7 | 1 | 2 | PMSF | 1 (mM) | |
| 8 | 1 | 2 | low concentration |
