1EQZ

X-RAY STRUCTURE OF THE NUCLEOSOME CORE PARTICLE AT 2.5 A RESOLUTION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1996-04-18
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	105.280, 109.710, 181.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.340 - 2.500
R-factor	0.219
Rwork	0.219
R-free	0.28200
Structure solution method	SIR, MR
Starting model (for MR)	2hio 1aoi
RMSD bond length	0.013
RMSD bond angle	20.500 *
Data scaling software	SCALEPACK
Phasing software	CCP4
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	25.000 *
High resolution limit [Å]	2.500
Rmerge	0.045 *
Total number of observations	605950 *
Number of reflections	72200 *
Completeness [%]	98.7
Redundancy	8.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6		pH 6.0, VAPOR DIFFUSION, HANGING DROP

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1		50 (mM)
2	1	1	potassium cacodylate	10 (mM)
3	1	1	PMSF	1 (mM)
4	1	1			high concentration
5	1	2		50 (mM)
6	1	2	potassium cacodylate	10 (mM)
7	1	2	PMSF	1 (mM)
8	1	2			low concentration