1EQV
SIMPLIFICATION OF A PROTEIN LOOP IN STAPHYLOCOCCAL NUCLEASE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 278 |
Detector technology | IMAGE PLATE |
Collection date | 1994-12-01 |
Detector | MACSCIENCE |
Spacegroup name | P 41 |
Unit cell lengths | 47.390, 47.390, 62.750 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 1.940 |
R-factor | 0.192 |
Rwork | 0.192 |
RMSD bond length | 0.010 |
RMSD bond angle | 24.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 6.000 | 2.060 |
High resolution limit [Å] | 1.940 | 1.940 |
Rmerge | 0.074 | |
Number of reflections | 8600 | |
Completeness [%] | 86.1 | 70 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | SMALL TUBES | 8.15 | 293 | 10.5 mM potassium phosphate, MPD, pH 8.15, SMALL TUBES, temperature 293K |
1 | SMALL TUBES | 8.15 | 293 | 10.5 mM potassium phosphate, MPD, pH 8.15, SMALL TUBES, temperature 293K |