1EQT
MET-RANTES
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-01-23 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 24.019, 56.809, 93.863 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.600 |
| R-factor | 0.178 |
| Rwork | 0.187 |
| R-free | 0.25130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | RANTES (NMR MODEL 1RTN) |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.024 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.039 | 0.420 |
| Total number of observations | 44155 * | |
| Number of reflections | 17136 | |
| <I/σ(I)> | 21.3 | 2.3 |
| Completeness [%] | 96.6 | 96 |
| Redundancy | 2.6 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | other * | 4.6 | 20 * | Wilken, J., (1999) Chem.Biol., 6, 43. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | sodium acetate | 10 (mM) | |
| 10 | 1 | 2 | ethanol | 15 (%) | |
| 2 | 1 | 1 | protein | 15 (mg/ml) | |
| 3 | 1 | 1 | ammonium sulfate | 0.1 (M) | |
| 4 | 1 | 1 | sodium succinate | 225 (mM) | |
| 5 | 1 | 1 | MES | 275 (mM) | |
| 6 | 1 | 1 | ethanol | 15 (%) | |
| 7 | 1 | 2 | ammonium sulfate | 0.1 (M) | |
| 8 | 1 | 2 | sodium succinate | 225 (mM) | |
| 9 | 1 | 2 | MES | 275 (mM) |
