1EQF

CRYSTAL STRUCTURE OF THE DOUBLE BROMODOMAIN MODULE FROM HUMAN TAFII250

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	1999-09-07
Detector	ADSC QUANTUM 4
Spacegroup name	P 21 21 21
Unit cell lengths	48.100, 58.100, 101.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.100 - 2.100
R-factor	0.218
Rwork	0.218
R-free	0.24600
RMSD bond length	0.009
RMSD bond angle	1.140
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MLPHARE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.300	29.100
High resolution limit [Å]	2.100	2.100
Rmerge	0.079	0.341
Total number of observations	122732 *
Number of reflections	17084
<I/σ(I)>	17.3
Completeness [%]	97.0	97.6
Redundancy	7.2	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	9.5	294	ammonium sulfate, TrisCl, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	10 (mg/ml)
2	1	1	ammonium sulfate	1.4 (M)
3	1	1	Tris-HCl	100 (mM)