1EQC

EXO-B-(1,3)-GLUCANASE FROM CANDIDA ALBICANS IN COMPLEX WITH CASTANOSPERMINE AT 1.85 A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1997-06-16
Detector	RIGAKU RAXIS II
Spacegroup name	P 21 21 21
Unit cell lengths	60.030, 65.120, 97.040
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.850
R-factor	0.157
Rwork	0.156
R-free	0.18600
RMSD bond length	0.011
RMSD bond angle	0.021
Data reduction software	ROTAVATA
Data scaling software	CCP4 ((AGROVATA)
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.000	1.950
High resolution limit [Å]	1.850	1.850
Rmerge	0.039	0.170
Number of reflections	32957
<I/σ(I)>	15.3
Completeness [%]	99.3	96.9
Redundancy	5.3	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4 *	291	HEPES BUFFER, CACL2, PEG8000, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	HEPES	50 (mM)
3	1	reservoir		0.2 (M)
4	1	reservoir	PEG8000	20 (%(w/v))