1EQ6

1.9 ANGSTROM RESOLUTION CRYSTAL STRUCTURE OF THE SACCHAROMYCES CEREVISIAE RAN-BINDING PROTEIN MOG1P

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID2
Synchrotron site	ESRF
Beamline	ID2
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-05-15
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	39.672, 51.963, 112.234
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.900
R-factor	0.2185
Rwork	0.217
R-free	0.24580
Structure solution method	MAD PLUS SIR
RMSD bond length	0.011
RMSD bond angle	25.320 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.039	0.146 *
Number of reflections	18625
<I/σ(I)>	11.5	4.9
Completeness [%]	98.3	98.7 *
Redundancy	3.5	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.6 *	291	Baker, R.P.,(2000) Acta Crystallog. Sect., D56, 229. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium acetate	0.2 (M)
2	1	reservoir	sodium citrate	1 (M)
3	1	reservoir	PEG4000	30 (%)
4	1	drop	protein	10-12 (mg/ml)