1EPY
T4 LYSOZYME MUTANT, T21H/C54T/C97A/Q141H/T142H
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1997-10-11 |
| Detector | XUONG-HAMLIN MULTIWIRE |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 62.385, 62.385, 97.241 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.850 |
| R-factor | 0.183 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.400 |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.800 * |
| Number of reflections | 19318 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 12-16% PEG 8000, 10% isopropanol, sodium chloride, HEPES, 0.002 M cobalt chloride, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 4K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 1 (mM) | |
| 2 | 1 | drop | HEPES | 20 (mM) | |
| 3 | 1 | reservoir | 200-300 (mM) | ||
| 4 | 1 | reservoir | HEPES | 20 (mM) | |
| 5 | 1 | reservoir | PEG8000 | 12-16 (%) | |
| 6 | 1 | reservoir | 2-propanol | 10 (%) | |
| 7 | 1 | reservoir | metal ion | 2 (mM) | |
| 8 | 1 | drop | Co(II) |
