1EP9
HUMAN ORNITHINE TRANSCARBAMYLASE: CRYSTALLOGRAPHIC INSIGHTS INTO SUBSTRATE RECOGNITION AND CONFORMATIONAL CHANGE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 298 |
Detector technology | AREA DETECTOR |
Collection date | 1999-08-27 |
Detector | SIEMENS |
Spacegroup name | P 21 3 |
Unit cell lengths | 125.600, 125.600, 125.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.400 |
Rwork | 0.184 |
R-free | 0.22300 |
RMSD bond length | 0.011 |
RMSD bond angle | 2.600 * |
Data reduction software | X-GEN |
Data scaling software | X-GEN |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.843) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.540 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.111 | 0.402 |
Total number of observations | 333653 * | |
Number of reflections | 26061 | |
<I/σ(I)> | 23.3 | |
Completeness [%] | 98.4 | 93.2 |
Redundancy | 12.8 | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 18 * | 20 MM TRISAC, 2MM EDTA, 20MM KCL, 4MM PALO, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | drop | PALO | 4 (mM) | |
3 | 1 | drop | Tris acetate | 20 (mM) | |
4 | 1 | drop | EDTA | 2 (mM) | |
5 | 1 | drop | 20 (mM) | ||
6 | 1 | reservoir | PEG400 | 2 (%) | |
7 | 1 | reservoir | ammonium sulfate | 2 (M) | |
8 | 1 | reservoir | sodium HEPES | 0.1 (M) |