1EP2
CRYSTAL STRUCTURE OF LACTOCOCCUS LACTIS DIHYDROOROTATE DEHYDROGENASE B COMPLEXED WITH OROTATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 288 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-01-31 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 202.420, 202.420, 80.880 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.500 - 2.400 |
| R-factor | 0.19 * |
| Rwork | 0.190 |
| R-free | 0.24100 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.639 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.500 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.099 | 0.497 |
| Total number of observations | 89776 * | |
| Number of reflections | 21335 | |
| <I/σ(I)> | 12.4 | |
| Completeness [%] | 86.0 | 90.1 |
| Redundancy | 4.2 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 * | 298 | Rowland, P., (1997) Acta Crystallogr., Sect.D, 53, 802. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | sodium phosphate | 50 (mM) | |
| 3 | 1 | drop | glycerol | 10 (%) | |
| 4 | 1 | reservoir | ammonium sulfate | 2.4 (M) | |
| 5 | 1 | reservoir | sodium acetate | 0.1 (M) |
