1EOW

CRYSTAL STRUCTURE OF RIBONUCLEASE A COMPLEXED WITH URIDYLYL(2',5')GUANOSINE (NON-PRODUCTIVE BINDING)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR591
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1998-04-20
Detector	MAC Science DIP-2030B
Spacegroup name	P 1 21 1
Unit cell lengths	30.340, 38.350, 53.850
Unit cell angles	90.00, 106.80, 90.00

Refinement procedure

Resolution	8.000 - 2.000
R-factor	0.187
Rwork	0.187
RMSD bond length	0.013
RMSD bond angle	24.000 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.068	0.112
Total number of observations	60998 *
Number of reflections	10866
<I/σ(I)>	14.1
Completeness [%]	96.4	92.7
Redundancy	5.6	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	FREE INTERFACE DIFFUSION	5.3 *	20 *	Berisio, R., (1999) J. Mol. Biol., 292, 845. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	30 (mg/ml)
2	1	2	2-methyl-2-propanol	50 (%)