1EOV

FREE ASPARTYL-TRNA SYNTHETASE (ASPRS) (E.C. 6.1.1.12) FROM YEAST

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	110
Detector technology	CCD
Collection date	1999-02-09
Detector	ADSC QUANTUM
Spacegroup name	P 41 21 2
Unit cell lengths	90.230, 90.230, 184.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.300
R-factor	0.202
Rwork	0.202
R-free	0.24200
Structure solution method	MR
Starting model (for MR)	1asz
RMSD bond length	0.009
RMSD bond angle	23.100 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.350
High resolution limit [Å]	2.300	2.300
Rmerge	0.082 *	0.277 *
Number of reflections	34124
<I/σ(I)>	12	4.6
Completeness [%]	98.2	99.3
Redundancy	5.4	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	278	15 MG/ML PROTEIN, 1.9 M AMMONIUM SULFATE, pH 5.60, VAPOR DIFFUSION, SITTING DROP, temperature 278.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	14 (mg/ml)
2	1	reservoir	ammonium sulfate	2.0 (M)