1EOS

CRYSTAL STRUCTURE OF RIBONUCLEASE A COMPLEXED WITH URIDYLYL(2',5')GUANOSINE (PRODUCTIVE BINDING)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR591
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1998-06-08
Detector	MAC Science DIP-2030B
Spacegroup name	C 1 2 1
Unit cell lengths	101.440, 33.450, 73.110
Unit cell angles	90.00, 90.50, 90.00

Refinement procedure

Resolution	8.000 - 2.000
Rwork	0.178
RMSD bond length	0.013
RMSD bond angle	23.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.082	0.360
Total number of observations	37174 *
Number of reflections	15833
<I/σ(I)>	10.3
Completeness [%]	92.2	83.7
Redundancy	2.4	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	free interface diffusion *	5.3 *	20 *	Berisio, R., (1999) J. Mol. Biol., 292, 845. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	30 (mg/ml)
2	1	2	2-methyl-2-propanol	50 (%)