1EOP
ECORV BOUND TO COGNATE DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 297 |
Detector technology | IMAGE PLATE |
Collection date | 1997-03-09 |
Detector | RIGAKU RAXIS II |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 66.100, 66.100, 299.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 4.800 - 2.600 |
R-factor | 0.187 |
Rwork | 0.181 |
R-free | 0.30200 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.800 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | |
High resolution limit [Å] | 2.600 | |
Rmerge | 0.103 | |
Total number of observations | 96208 * | |
Number of reflections | 18023 | |
<I/σ(I)> | 4 | |
Completeness [%] | 78.0 | 50 * |
Redundancy | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.5 * | 290 | drop consists of equal volume of protein and reservoir solutions * |
1 | Vapor diffusion * | 7.5 * | 290 | drop consists of equal volume of protein and reservoir solutions * |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | acetate | ||
2 | 1 | 1 | tartarate | ||
3 | 1 | 1 | PEG 4000 | ||
4 | 1 | 2 | PEG 4000 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | EcoRV | 10 (mg/ml) | |
2 | 1 | drop | DNA | 1.5-fold molar excess | |
3 | 1 | reservoir | acetate | 0.1 (M) | |
4 | 1 | reservoir | Na/K-tartrate | 0.2 (M) | |
5 | 1 | reservoir | PEG4000 | 10 (%) |