1EOP
ECORV BOUND TO COGNATE DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 297 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-03-09 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 66.100, 66.100, 299.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 4.800 - 2.600 |
| R-factor | 0.187 |
| Rwork | 0.181 |
| R-free | 0.30200 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 2.600 | |
| Rmerge | 0.103 | |
| Total number of observations | 96208 * | |
| Number of reflections | 18023 | |
| <I/σ(I)> | 4 | |
| Completeness [%] | 78.0 | 50 * |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 * | 290 | drop consists of equal volume of protein and reservoir solutions * |
| 1 | Vapor diffusion * | 7.5 * | 290 | drop consists of equal volume of protein and reservoir solutions * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | acetate | ||
| 2 | 1 | 1 | tartarate | ||
| 3 | 1 | 1 | PEG 4000 | ||
| 4 | 1 | 2 | PEG 4000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | EcoRV | 10 (mg/ml) | |
| 2 | 1 | drop | DNA | 1.5-fold molar excess | |
| 3 | 1 | reservoir | acetate | 0.1 (M) | |
| 4 | 1 | reservoir | Na/K-tartrate | 0.2 (M) | |
| 5 | 1 | reservoir | PEG4000 | 10 (%) |
