1EOP

ECORV BOUND TO COGNATE DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	297
Detector technology	IMAGE PLATE
Collection date	1997-03-09
Detector	RIGAKU RAXIS II
Spacegroup name	P 41 21 2
Unit cell lengths	66.100, 66.100, 299.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	4.800 - 2.600
R-factor	0.187
Rwork	0.181
R-free	0.30200
RMSD bond length	0.011
RMSD bond angle	1.800
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.600
Rmerge	0.103
Total number of observations	96208 *
Number of reflections	18023
<I/σ(I)>	4
Completeness [%]	78.0	50 *
Redundancy	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5 *	290	drop consists of equal volume of protein and reservoir solutions *
1	Vapor diffusion *	7.5 *	290	drop consists of equal volume of protein and reservoir solutions *

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	acetate
2	1	1	tartarate
3	1	1	PEG 4000
4	1	2	PEG 4000

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	EcoRV	10 (mg/ml)
2	1	drop	DNA		1.5-fold molar excess
3	1	reservoir	acetate	0.1 (M)
4	1	reservoir	Na/K-tartrate	0.2 (M)
5	1	reservoir	PEG4000	10 (%)