1EOO
ECORV BOUND TO COGNATE DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL1-5 |
| Synchrotron site | SSRL |
| Beamline | BL1-5 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-04-19 |
| Detector | OTHER |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 72.600, 120.400, 182.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 4.800 - 2.160 |
| R-factor | 0.2285 |
| Rwork | 0.231 |
| R-free | 0.31600 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.100 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.220 |
| High resolution limit [Å] | 2.160 | 2.160 |
| Rmerge | 0.048 | 0.315 |
| Total number of observations | 50635 * | |
| Number of reflections | 21638 | |
| <I/σ(I)> | 9.1 | |
| Completeness [%] | 82.0 | 81.5 |
| Redundancy | 1.8 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 * | 297 | drop consists of equal volume of protein and reservoir solutions * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | (NH4)2SO4 | ||
| 2 | 1 | 2 | (NH4)2SO4 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | EcoRV | 10 (mg/ml) | |
| 2 | 1 | drop | DNA | 1.5-fold molar excess | |
| 3 | 1 | reservoir | ammonium sulfate | 1.5 (M) |
