1EOF

CRYSTAL STRUCTURE OF THE N136A MUTANT OF A SHAKER T1 DOMAIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	MACSCIENCE
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-02-09
Detector	MACSCIENCE
Spacegroup name	I 4 2 2
Unit cell lengths	59.384, 59.384, 146.894
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.900 - 2.380
R-factor	0.225
Rwork	0.225
R-free	0.26400
RMSD bond length	0.009
RMSD bond angle	3.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	2.460
High resolution limit [Å]	2.380	2.380
Rmerge	0.069	0.256
Total number of observations	81032 *
Number of reflections	5586
<I/σ(I)>	11.9
Completeness [%]	98.0 *	100
Redundancy	14	8.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5 *	277	Bixby, K.A., (1999) Nature Struct. Biol., 6, 38. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	isopropanol	24 (%)
2	1	reservoir		0.2 (M)
3	1	reservoir	HEPES	0.1 (M)
4	1	reservoir	dithiothreitol	1 (mM)