1EOE

CRYSTAL STRUCTURE OF THE V135R MUTANT OF A SHAKER T1 DOMAIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	1999-09-27
Detector	ADSC QUANTUM 4
Spacegroup name	I 4 2 2
Unit cell lengths	60.138, 60.138, 146.793
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.840 - 1.704
R-factor	0.21854
Rwork	0.208
R-free	0.24930
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.078	0.234
Total number of observations	130025 *
Number of reflections	15106
<I/σ(I)>	13.5
Completeness [%]	92.2	80.7
Redundancy	8	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5	277	Bixby, K.A., (1999) Nature Struct. Biol., 6, 38. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	isopropanol	24 (%)
2	1	reservoir		0.2 (M)
3	1	reservoir	HEPES	0.1 (M)
4	1	reservoir	dithiothreitol	1 (mM)