1EOD

CRYSTAL STRUCTURE OF THE N136D MUTANT OF A SHAKER T1 DOMAIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	MACSCIENCE
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-02-09
Detector	MACSCIENCE
Spacegroup name	I 4 2 2
Unit cell lengths	59.464, 59.464, 146.889
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.910 - 2.450
R-factor	0.211
Rwork	0.211
R-free	0.23400
RMSD bond length	0.008
RMSD bond angle	2.700
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.540
High resolution limit [Å]	2.450	2.450
Rmerge	0.071	0.245
Total number of observations	76332 *
Number of reflections	5159
<I/σ(I)>	11.2
Completeness [%]	99.2	99.2
Redundancy	14	8.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5	277	Bixby, K.A., (1999) Nature Struct. Biol., 6, 38. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	isopropanol	24 (%)
2	1	reservoir		0.2 (M)
3	1	reservoir	HEPES	0.1 (M)
4	1	reservoir	dithiothreitol	1 (mM)