1EO5
Bacillus circulans strain 251 cyclodextrin glycosyltransferase in complex with maltoheptaose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-06-23 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 116.700, 109.700, 67.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.000 |
R-factor | 0.167 * |
Rwork | 0.167 |
R-free | 0.21000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1cxi |
RMSD bond length | 0.006 |
RMSD bond angle | 0.652 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | TNT |
Refinement software | TNT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 79.900 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.111 | 0.414 |
Number of reflections | 54766 | |
<I/σ(I)> | 7.72 | 2.9 |
Completeness [%] | 92.1 | 88.1 |
Redundancy | 3.48 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 8.1 * | Lawson, C.L., (1994) J. Mol. Biol., 236, 590. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | MPD | 60 (%(v/v)) | |
2 | 1 | reservoir | Tris-HCl | 100 (mM) | |
3 | 1 | reservoir | maltose | 5 (%(w/v)) |