1ENV
ATOMIC STRUCTURE OF THE ECTODOMAIN FROM HIV-1 GP41
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-13 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-02 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 52.350, 52.350, 414.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 2.600 |
| R-factor | 0.244 |
| Rwork | 0.244 |
| R-free | 0.27700 |
| Structure solution method | MIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.590 |
| Rmerge | 0.071 | 0.179 |
| Number of reflections | 8130 | |
| <I/σ(I)> | 10.2 | 4.5 |
| Completeness [%] | 90.5 | 91.4 |
| Redundancy | 7.45 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 | 20 MM HEPES PH 8.0 1.1 M AMMONIUM SULFATE 12% ETHYLENE GLYCOL SPACE GROUP R32 IN HEXAGONAL SETTING |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15-20 (mg/ml) | |
| 2 | 1 | drop | HEPES | 20 (mg/ml) | |
| 3 | 1 | drop | 75 (mM) | ||
| 4 | 1 | reservoir | HEPES | 100 (mM) | |
| 5 | 1 | reservoir | ammonium sulfate | 1.1 (M) | |
| 6 | 1 | reservoir | ethylene glycol | 12 (%) |
