1ENO

BRASSICA NAPUS ENOYL ACP REDUCTASE/NAD BINARY COMPLEX AT PH 8.0 AND ROOM TEMPERATURE

Experimental procedure

Source type	ROTATING ANODE
Detector technology	AREA DETECTOR
Collection date	1993-10-15
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	P 42 21 2
Unit cell lengths	70.500, 70.500, 117.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.900
R-factor	0.164 *
RMSD bond length	0.017
RMSD bond angle	2.000
Data reduction software	SDMS
Refinement software	TNT

Data quality characteristics

	Overall
High resolution limit [Å]	1.900 *
Rmerge	0.047
Total number of observations	93536 *
Number of reflections	20642
Completeness [%]	83.0
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.2 *	17 *	Rafferty, J.B., (1994) J.Mol.Biol., 237, 240. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	10 (mg/ml)
2	1	drop		10 (mM)
3	1	drop	dithiothreitol	1 (mM)
4	1	reservoir	nucleotide	0.15 (M)
5	1	reservoir	ammonium sulfate	2.0 (M)
6	1	reservoir	HEPES/NaOH	0.1 (M)