1ENH

STRUCTURAL STUDIES OF THE ENGRAILED HOMEODOMAIN

Experimental procedure

Spacegroup name	P 65 2 2
Unit cell lengths	44.670, 44.670, 118.120
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.100
R-factor	0.197
Rwork	0.197
RMSD bond length	0.013
RMSD bond angle	1.590 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.890 *
Rmerge	0.037 *
Total number of observations	21567 *
Number of reflections	6104 *
Completeness [%]	86.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.7 *		referred to 'Liu B.', (1990) 'Biochem. Biophys. Res. Commun.', 171, 257-259 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	4.5 (mg/ml)
2	1	drop	DNA		duplex
3	1	drop	bis-Tris-HCl	30 (mM)
4	1	reservoir	ammonium phosphate	74 (%sat)