1EMY

CRYSTAL STRUCTURE OF ASIAN ELEPHANT (ELEPHAS MAXIMUS) CYANO-MET MYOGLOBIN AT 1.78 ANGSTROMS RESOLUTION. PHE 29 (B10) ACCOUNTS FOR ITS UNUSUAL LIGAND BINDING PROPERTIES

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	33.510, 58.590, 70.440
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.780
R-factor	0.153
Rwork	0.153
RMSD bond length	0.019
RMSD bond angle	0.039 *
Phasing software	X-PLOR
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	1.780 *
Rmerge	0.045 *
Total number of observations	68768 *
Number of reflections	13649
Completeness [%]	93.6
Redundancy	5.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5 *		macroseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG1000	45 (%)
3	1	reservoir	Tris-HCl	100 (mM)
4	1	reservoir	magnesium acetate	150 (mM)