1EMV
CRYSTAL STRUCTURE OF COLICIN E9 DNASE DOMAIN WITH ITS COGNATE IMMUNITY PROTEIN IM9 (1.7 ANGSTROMS)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-09-19 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.870, 52.560, 87.770 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.700 |
R-factor | 0.219 * |
Rwork | 0.219 |
R-free | 0.26000 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.538 |
Data reduction software | DENZO |
Data scaling software | CCP4 ((TRUNCATE)) |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.740 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.026 | 0.071 |
Total number of observations | 415140 * | |
Number of reflections | 20871 | |
<I/σ(I)> | 47.1 | |
Completeness [%] | 89.9 | 73.4 |
Redundancy | 19.8 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.3 | 277 | used seeding, Kuhlmann, U.C., (1999) Acta. Crystallog. sect. D55, 256. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG4000 | 32 (%(w/v)) | |
2 | 1 | reservoir | sodium acetate | 0.1 (M) |