1EMU
STRUCTURE OF THE AXIN RGS-HOMOLOGOUS DOMAIN IN COMPLEX WITH A SAMP REPEAT FROM APC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-02-02 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 30.600, 69.980, 71.960 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.000 - 1.900 |
| R-factor | 0.205 |
| Rwork | 0.205 |
| R-free | 0.22100 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 18.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.050 | 0.295 |
| Number of reflections | 12617 | |
| <I/σ(I)> | 19.3 | |
| Completeness [%] | 99.3 | 99.7 |
| Redundancy | 3.2 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 4 * | MES, PEG 400, glycerol, DTT, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 4 * | MES, PEG 400, glycerol, DTT, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Axin-RGS | 1 (mM) | |
| 2 | 1 | drop | SAMP3 | 1 (mM) | |
| 3 | 1 | reservoir | MES | 50 (mM) | |
| 4 | 1 | reservoir | PEG400 | 42.5 (%) | |
| 5 | 1 | reservoir | glycerol | 15 (%) | |
| 6 | 1 | reservoir | dithiothreitol | 5 (mM) |
