Loading
PDBj
MenuPDBj@FacebookPDBj@TwitterPDBj@YouTubewwPDB FoundationwwPDB
RCSB PDBPDBeBMRBAdv. SearchSearch help

1EMG

GREEN FLUORESCENT PROTEIN (65-67 REPLACED BY CRO, S65T SUBSTITUTION, Q80R)

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeROTATING ANODE
Source detailsRIGAKU RUH3R
Temperature [K]295
Detector technologyIMAGE PLATE
DetectorRIGAKU RAXIS IV
Spacegroup nameP 21 21 21
Unit cell lengths51.733, 62.822, 70.517
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution20.000 - 2.000
R-factor0.19

*

Rwork0.186
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1ema
RMSD bond length0.009
RMSD bond angle2.100
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareTNT
Refinement softwareTNT
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]20.0002.050
High resolution limit [Å]2.0002.000
Rmerge0.075
Total number of observations93073

*

Number of reflections16011
Completeness [%]99.094
Redundancy4
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1Vapor diffusion, hanging drop

*

7.9

*

CRYSTALLIZATION CONDITIONS: 22-26% PEG 4000, 50 MM HEPES PH 8.0, 50 MM MGCL2, 12 MG PROTEIN
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropprotein12 (mg/ml)
21dropTris20 (mM)
31reservoirHEPES50 (mM)
41reservoir50 (mM)
51reservoirPEG400023-26 (%)

217705

PDB entries from 2024-03-27

PDB statisticsPDBj update infoContact PDBjnumon