1EME
GREEN FLUORESCENT PROTEIN FROM AEQUOREA VICTORIA, MUTANT
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 295 |
Detector technology | IMAGE PLATE |
Collection date | 1996-03-05 |
Detector | MAC Science DIP-2000 |
Spacegroup name | I 41 3 2 |
Unit cell lengths | 175.600, 175.600, 175.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.500 |
R-factor | 0.198 |
Rwork | 0.198 |
R-free | 0.28500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ema |
RMSD bond length | 0.019 |
RMSD bond angle | 28.930 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.540 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.094 * | 0.291 * |
Number of reflections | 15816 | |
<I/σ(I)> | 6.5 | 2.9 |
Completeness [%] | 97.4 | 94.7 |
Redundancy | 9.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | protein solution is mixed in a 1:1 ratio with well solution * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 13 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 20 (mM) | |
3 | 1 | reservoir | ammonium sulfate | 1.8 (M) | |
4 | 1 | reservoir | Tris-HCl | 100 (mM) |