Loading
PDBj
MenuPDBj@FacebookPDBj@TwitterPDBj@YouTubewwPDB FoundationwwPDB
RCSB PDBPDBeBMRBAdv. SearchSearch help

1EME

GREEN FLUORESCENT PROTEIN FROM AEQUOREA VICTORIA, MUTANT

Experimental procedure
Source typeROTATING ANODE
Source detailsRIGAKU RUH2R
Temperature [K]295
Detector technologyIMAGE PLATE
Collection date1996-03-05
DetectorMAC Science DIP-2000
Spacegroup nameI 41 3 2
Unit cell lengths175.600, 175.600, 175.600
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution10.000 - 2.500
R-factor0.198
Rwork0.198
R-free0.28500
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1ema
RMSD bond length0.019
RMSD bond angle28.930

*

Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareX-PLOR (3.1)
Refinement softwareX-PLOR (3.1)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]20.0002.540
High resolution limit [Å]2.5002.500
Rmerge0.094

*

0.291

*

Number of reflections15816
<I/σ(I)>6.52.9
Completeness [%]97.494.7
Redundancy9.4
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP8.5protein solution is mixed in a 1:1 ratio with well solution

*

Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropprotein13 (mg/ml)
21dropTris-HCl20 (mM)
31reservoirammonium sulfate1.8 (M)
41reservoirTris-HCl100 (mM)

217705

PDB entries from 2024-03-27

PDB statisticsPDBj update infoContact PDBjnumon