1EMC
GREEN FLUORESCENT PROTEIN FROM AEQUOREA VICTORIA, MUTANT
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-10-12 |
| Detector | MACSCIENCE |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 93.000, 52.000, 103.000 |
| Unit cell angles | 90.00, 101.80, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.300 |
| R-factor | 0.205 |
| Rwork | 0.205 |
| R-free | 0.28800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ema |
| RMSD bond length | 0.019 |
| RMSD bond angle | 28.530 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.078 * | 0.253 * |
| Number of reflections | 31013 | |
| <I/σ(I)> | 6.5 | 1.9 |
| Completeness [%] | 78.5 | 56.9 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | protein solution is mixed in a 1:1 ratio with well solution * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 14 (mg/ml) | |
| 2 | 1 | drop | 20 (mM) | ||
| 3 | 1 | reservoir | MPD | 60 (%) | |
| 4 | 1 | reservoir | Tris-HCl | 50 (mM) |
