1EMB
GREEN FLUORESCENT PROTEIN (GFP) FROM AEQUOREA VICTORIA, GLN 80 REPLACED WITH ARG
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-09 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.900, 63.200, 68.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.130 |
| R-factor | 0.196 * |
| Rwork | 0.196 |
| R-free | 0.25400 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ema |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.534 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.200 |
| High resolution limit [Å] | 2.130 | 2.130 |
| Rmerge | 0.061 * | 0.186 * |
| Total number of observations | 60182 * | |
| Number of reflections | 13001 | |
| <I/σ(I)> | 7.1 | 3.9 |
| Completeness [%] | 91.3 | 54.5 |
| Redundancy | 4.8 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 8 * | PROTEIN WAS CRYSTALLIZED FROM 50 MM KH2PO4 AND 20 % (W/V) PEG 8000, PH 3.8. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | GFP | 11 (mg/ml) | |
| 2 | 1 | drop | Tris | 7 (mM) | |
| 3 | 1 | drop | 0.01 (%) | ||
| 4 | 1 | drop | 0.01 (M) | ||
| 5 | 1 | drop | PEG8000 | 0.03 (%(w/v)) | |
| 6 | 1 | reservoir | 0.05 (M) | ||
| 7 | 1 | reservoir | PEG8000 | 20 (%(w/v)) |
