1EM8
Crystal structure of chi and psi subunit heterodimer from DNA POL III
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-01-01 |
| Detector | RIGAKU |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.377, 65.719, 73.429 |
| Unit cell angles | 90.00, 116.15, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.233 |
| Rwork | 0.229 |
| R-free | 0.26500 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 3.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.066 | 0.194 |
| Total number of observations | 218771 * | |
| Number of reflections | 32326 * | |
| <I/σ(I)> | 13.2 | |
| Completeness [%] | 97.9 | 95.2 |
| Redundancy | 6.8 | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 4 * | methylpentanediol, HEPES, PEG 4000, Glycerol, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | Tris-HCl | 20 (mM) | pH7.5 |
| 3 | 1 | reservoir | dithiothreitol | 4 (mM) | |
| 4 | 1 | reservoir | EDTA | 0.5 (mM) | |
| 5 | 1 | reservoir | 100 (mM) | ||
| 6 | 1 | reservoir | glycerol | 10 (%(v/v)) |
