1EM8

Crystal structure of chi and psi subunit heterodimer from DNA POL III

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1994-01-01
Detector	RIGAKU
Spacegroup name	P 1 21 1
Unit cell lengths	64.377, 65.719, 73.429
Unit cell angles	90.00, 116.15, 90.00

Refinement procedure

Resolution	20.000 - 2.100
R-factor	0.233
Rwork	0.229
R-free	0.26500
RMSD bond length	0.010
RMSD bond angle	3.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.200
High resolution limit [Å]	2.100	2.100
Rmerge	0.066	0.194
Total number of observations	218771 *
Number of reflections	32326 *
<I/σ(I)>	13.2
Completeness [%]	97.9	95.2
Redundancy	6.8	1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	4 *	methylpentanediol, HEPES, PEG 4000, Glycerol, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	Tris-HCl	20 (mM)	pH7.5
3	1	reservoir	dithiothreitol	4 (mM)
4	1	reservoir	EDTA	0.5 (mM)
5	1	reservoir		100 (mM)
6	1	reservoir	glycerol	10 (%(v/v))