1EM6
HUMAN LIVER GLYCOGEN PHOSPHORYLASE A COMPLEXED WITH GLCNAC AND CP-526,423
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-04-18 |
| Detector | BRANDEIS - B4 |
| Spacegroup name | P 31 |
| Unit cell lengths | 123.314, 123.314, 122.319 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 99.000 - 2.200 |
| R-factor | 0.234 |
| Rwork | 0.233 |
| R-free | 0.26400 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 * | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.074 | 0.359 |
| Number of reflections | 103325 | |
| <I/σ(I)> | 13.6 | |
| Completeness [%] | 97.8 | 87.1 |
| Redundancy | 2.4 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6 | 290 | MES, MPD, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
