1EM2
Star-related lipid transport domain of MLN64
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 1999-11-16 |
Detector | OTHER |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 83.417, 83.417, 81.946 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.000 - 2.200 |
R-factor | 0.207 * |
Rwork | 0.207 |
R-free | 0.26400 |
RMSD bond length | 0.017 * |
RMSD bond angle | 1.850 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 25.000 |
High resolution limit [Å] | 2.200 |
Rmerge | 0.065 |
Total number of observations | 136560 * |
Number of reflections | 16818 * |
Completeness [%] | 98.1 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 24 * | K/Na tartrare, lithium sulfate, CHES, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | CHES | 100 (mM) | pH9.5 |
2 | 1 | reservoir | sodium potassium tartrate | 950 (mM) | |
3 | 1 | reservoir | 200 (mM) |