1ELU
COMPLEX BETWEEN THE CYSTINE C-S LYASE C-DES AND ITS REACTION PRODUCT CYSTEINE PERSULFIDE.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-08-07 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.448, 65.386, 170.532 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 1.550 |
R-factor | 0.214 * |
Rwork | 0.214 |
R-free | 0.25900 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.580 |
Data reduction software | DENZO |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.570 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.052 | 0.365 |
Total number of observations | 224370 * | |
Number of reflections | 95716 | |
<I/σ(I)> | 18.5 | |
Completeness [%] | 93.7 | 87.3 |
Redundancy | 2.3 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 6.5 * | 20 * | PEG8000, ammonium sulfate, phosphate, citrate, barium chloride, cysteine, cystine, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | C-DES | 10 (mg/ml) | |
2 | 1 | drop | MOPS/NaOH | 10 (mM) | |
3 | 1 | drop | PLP | 0.010 (mM) | |
4 | 1 | reservoir | PEG8000 | 27 (%(v/v)) | |
5 | 1 | reservoir | ammonium sulfate | 0.1 (M) | |
6 | 1 | reservoir | citrate | 0.1 (M) |