1ELO
ELONGATION FACTOR G WITHOUT NUCLEOTIDE
Experimental procedure
Detector technology | AREA DETECTOR |
Detector | SIEMENS |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 75.600, 106.000, 116.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.800 |
R-factor | 0.23 |
Rwork | 0.230 |
R-free | 0.32000 |
RMSD bond length | 0.020 |
RMSD bond angle | 26.600 * |
Data reduction software | XENGEN |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
Completeness [%] | 87.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 4 * | Casale, E., (1991) J. Mol. Biol., 222, 447. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | reservoir | succinate | 0.05 (M) | |
3 | 1 | reservoir | ammonium sulfate | 3.3 (M) |