1ELL
CADMIUM-SUBSTITUTED BOVINE PANCREATIC CARBOXYPEPTIDASE A (ALFA-FORM) AT PH 7.5 AND 0.25 M CHLORIDE IN MONOCLINIC CRYSTAL FORM.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 288 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-12-18 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.294, 60.173, 51.752 |
| Unit cell angles | 90.00, 97.44, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.760 |
| R-factor | 0.167 |
| Rwork | 0.167 |
| R-free | 0.17810 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cpa |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 1.260 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.790 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.054 | 0.181 |
| Number of reflections | 233641 * | |
| <I/σ(I)> | 27.7 | 8.1 |
| Completeness [%] | 97.2 * | 79.7 |
| Redundancy | 7.3 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 7.5 | 293 | Lithium Chloride, Tris-HNO3, Cadmium Chloride, pH 7.5, MICRODIALYSIS, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | 1.0 (M) | ||
| 2 | 1 | 1 | Tris-HNO3 | 20 (mM) | pH7.5 |
| 3 | 1 | 1 | protein | 20 (mg/ml) | |
| 4 | 1 | 2 | 0.35 (M) | ||
| 5 | 1 | 2 | Tris-HNO3 | 20 (mM) | pH7.5 |
