1ELB

Analogous inhibitors of elastase do not always bind analogously

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	50.690, 58.050, 75.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.100
R-factor	0.15
Rwork	0.150
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.060 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5 *		THE COMPLEX WAS DONE BY SOAKING OF THE INHIBITOR. THE NATIVE ELASTASE CRYSTALS WERE TRANSFERRED FROM THE SITTING DROP TO A HIGH-SALT MOTHER LIQUOR CONTAINING THE TFA-LYS-PRO-ISO INHIBITOR AT A CONCENTRATION OF 0.1 MILLIMOLAR. THE INHIBITOR WAS SOAKED FOR FOUR DAYS.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	methanol	70 (%)
2	1	reservoir	acetate	30 (%)