1EL4
STRUCTURE OF THE CALCIUM-REGULATED PHOTOPROTEIN OBELIN DETERMINED BY SULFUR SAS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-09-18 |
| Detector | MARRESEARCH |
| Spacegroup name | P 62 |
| Unit cell lengths | 81.562, 81.562, 86.088 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.840 - 1.730 |
| R-factor | 0.189 * |
| Rwork | 0.188 |
| R-free | 0.20500 |
| Structure solution method | SULFUR SAS |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE (FOLLOWED BY ISAS) |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.790 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmerge | 0.038 | 0.216 |
| Number of reflections | 33058 * | |
| <I/σ(I)> | 37.6 | |
| Completeness [%] | 99.5 | 97.1 |
| Redundancy | 5.8 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 * | 277 | Vysotski, E.S., (1999) Acta Crystallogr., Sect.D, 55, 1965. * |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 * | 277 | Vysotski, E.S., (1999) Acta Crystallogr., Sect.D, 55, 1965. * |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 * | 277 | Vysotski, E.S., (1999) Acta Crystallogr., Sect.D, 55, 1965. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | potassium/sodium phosphate | 10 (mM) | |
| 2 | 1 | drop | EDTA | 1 (mM) | |
| 3 | 1 | drop | protein | 10 (mg/ml) | |
| 4 | 1 | reservoir | sodium citrate | 1.4 (M) |
