1EL1

X-RAY CRYSTAL STRUCTURE ANALYSIS OF CANINE MILK LYSOZYME (HOLO-TYPE)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-18B
Synchrotron site	Photon Factory
Beamline	BL-18B
Temperature [K]	277
Detector technology	DIFFRACTOMETER
Collection date	1999-01-01
Detector	WEISSENBERG
Spacegroup name	P 32
Unit cell lengths	31.300, 31.300, 202.500
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 1.900
Rwork	0.159
R-free	0.25700
RMSD bond length	0.006
RMSD bond angle	1.338
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.075	0.290
Number of reflections	16169
<I/σ(I)>	11.2
Completeness [%]	91.9	90.7
Redundancy	2.66

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	291	ammonium sulfate, sodium phosphate, calcium chloride, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 291K