1EKP
CRYSTAL STRUCTURE OF HUMAN BRANCHED CHAIN AMINO ACID AMINOTRANSFERASE (MITOCHONDRIAL) COMPLEXED WITH PYRIDOXAL-5'-PHOSPHATE AT 2.5 ANGSTROMS (MONOCLINIC FORM).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-03-22 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.630, 111.640, 59.900 |
| Unit cell angles | 90.00, 95.89, 90.00 |
Refinement procedure
| Resolution | 40.650 - 2.500 |
| R-factor | 0.166 |
| Rwork | 0.155 |
| R-free | 0.22000 * |
| Starting model (for MR) | E. COLI BRANCHED CHAIN AMINO ACID AMINOTRANSFERASE |
| RMSD bond length | 0.008 * |
| RMSD bond angle | 1.332 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.650 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.063 | 0.203 |
| Number of reflections | 21222 | |
| <I/σ(I)> | 13.1 | |
| Completeness [%] | 78.6 * | 66.8 |
| Redundancy | 2.632 | 1.35 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | PEG1500, DTT, HEPES; NA OR K PHOSPHATE, DTT, HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 2.5 (mg/ml) | |
| 2 | 1 | drop | HEPES | 50 (mm) | |
| 3 | 1 | drop | dithiothreitol | 20 (mM) | |
| 4 | 1 | drop | EDTA | 50 (mM) | |
| 5 | 1 | reservoir | sodium phosphate | 1.6 (M) | or potassium phosphate |
| 6 | 1 | reservoir | dithiothreitol | 20 (mM) | |
| 7 | 1 | reservoir | HEPES | 0.1 (M) |
