1EK8

CRYSTAL STRUCTURE OF THE RIBOSOME RECYCLING FACTOR (RRF) FROM ESCHERICHIA COLI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	1999-02-10
Detector	ADSC QUANTUM 4
Spacegroup name	P 31 2 1
Unit cell lengths	48.064, 48.064, 142.273
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.300
R-factor	0.228 *
Rwork	0.228
R-free	0.29700
RMSD bond length	1.634
RMSD bond angle	0.010
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.048	0.230
Total number of observations	29485 *
Number of reflections	8691	858 *
<I/σ(I)>	11
Completeness [%]	96.2	97.7
Redundancy	3.34	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	14 *	0.1 M MES-NaOH, 10 % PEG 350 MME, 12 % PEG 400, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 287K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	MES-NaOH	0.1 (M)
2	1	reservoir	PEG350 MME	10 (%)
3	1	reservoir	PEG400	12-14 (%)
4	1	drop	decyl-beta-D-maltopyranoside	1.8 (mM)