1EK8
CRYSTAL STRUCTURE OF THE RIBOSOME RECYCLING FACTOR (RRF) FROM ESCHERICHIA COLI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-02-10 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 48.064, 48.064, 142.273 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.228 * |
| Rwork | 0.228 |
| R-free | 0.29700 |
| RMSD bond length | 1.634 |
| RMSD bond angle | 0.010 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.048 | 0.230 |
| Total number of observations | 29485 * | |
| Number of reflections | 8691 | 858 * |
| <I/σ(I)> | 11 | |
| Completeness [%] | 96.2 | 97.7 |
| Redundancy | 3.34 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 14 * | 0.1 M MES-NaOH, 10 % PEG 350 MME, 12 % PEG 400, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 287K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | MES-NaOH | 0.1 (M) | |
| 2 | 1 | reservoir | PEG350 MME | 10 (%) | |
| 3 | 1 | reservoir | PEG400 | 12-14 (%) | |
| 4 | 1 | drop | decyl-beta-D-maltopyranoside | 1.8 (mM) |
